Maratha Mandal's Central Research Laboratory, Belgaum
Training modules

Maratha Mandal's Central Research Laboratory, Belgaum - Training modules


1. Anaerobic bacteriology
2. Molecular Biology
            a. PCR
            b. FISH
            c. LAMP
3. Cell culture
4. Immunology
            a. ELISA
            b. PAGE
            c. Western blotting
            d. Phagocytic function test
5. HPLC and Spectrophotometry
6. Extraction methods
7. Animal experiments

1. Sample collection
2. Transport medium
3. Processing samples
            a. Inoculation
            b. Incubation
            c. Identification
            d. Storage
4. Transport of cultures
5. MIC
6. Disc Diffusion Duration : 15 days
Cost : 15,000/-

    SAMPLE PROCESSING
                Sample Collection
                DNA/RNA extraction
                DNA/RNA quantification

    CONVENTIONAL PCR
                Mastermix Preparation
                Amplification
                Agarose Gel Electrophoresis
                Report and Analysis

    MULTIPLEX PCR
                Mastermix Preparation
                Amplification
                Agarose Gel Electrophoresis
                Report and Analysis

    REAL TIME PCR
                Mastermix Preparation
                SYBR Green assay

    LAMP
                Isothermal amplification
                Agarose gel electrophoresis
                Report and analysis

    FISH
                Sample collection and storage
                Slide preparation
                Strains
                Report and analysis


    Duration : 20 Days
    Cost : 20,000/-

1. Collection of samples
           Gingiva
           POL

2. Processing
3. MTT assay
4. Tryphan blue
5. Microscopic counting
6. Mitochondrial membrane potential
7. DAPI Staining

Duration : 10 Days
Cost : 10,000/-

1. ELISA
Using Commercial Kit
Duration : 6 Days
Cost : 5000/-


2. PAGE
Duration :6 Days
Cost : Rs 5000


3. Western Blotting
Duration :6 Days
Cost :Rs 7,500


4. Phagocytic function test
Duration : 6 Days
Cost :Rs 5000


5. Immunology Full Course
Duration : 15 Days
Cost :Rs 15,000




Duration : 6 Days
Cost : Rs 15000

1. Lyophilization
Duration :3 days
Cost : Rs 10,000


2. Novel drug delivery systems
Duration :15 days
Cost : Rs 10,000
Training slots :

Eligibility :

1. Animal maintenance
2. Animal Care and handling
3. Pharmacovigilance.


Duration : 6 Days
Cost : Rs 10,000