List of test performed in Maratha Mandal's Central Research
Laboratory, Belgaum
Sl. No
TESTS
Microbiology
1
Diffusion test for aerobic bacteria/facultative bacteria
2
Diffusion test for anaerobes
3
MIC activity for aerobic bacteria/ facultative bacteria
4
MIC activity for anaerobic bacteria
5
MBC activity for aerobic bacteria/facultative bacteria
6
MBC activity for anaerobic bacteria
Time Kill Curve
7
Aerobic/facultative bacteria for 4 exposure
8
Anerobic bacteria for 4 exposure
Synergism Studies (New)
9
Two drug combination
10
Three drug combination
Antifungal Assay
11
Diffusion test
12
MIC for fungi
13
MBC for fungi
14
Anti tubercular activity
Culture
Total facultative/Aerobic bacteria
15
Plate count
Culture for individual organisms
16
One Bacteria
17
Two Bacteria
18
Three Bacteria
19
More than three bacteria(New)
Anaerobic bacteria
20
Total Plate count
21
One Bacteria
22
Two Bacteria
23
Three Bacteria
24
More than three bacteria
25
Culture for Novel Bacteria not yet cultivable anaerobic bacteria
Biofilm studies
26
Cyrstal violet assay
27
Biofilm monospecies
28
Multi species
29
Biotic surface
30
Abiotic surface
Bioflim analysis
31
Culture
32
Double staining
33
Biofilm adhesion
34
Biofilm penetration
Immunology
35
ELISA
36
In-house ELISA
37
Gelatin Zymography
38
Western Blotting
39
Protein Blotting
40
PAGE
41
2-D Gel Electrophoresis
42
Protein Analysis
43
Immunofluoresence
44
Immunohistochemistry
45
Immunocytochemistry
Neutrophil function tests
46
Chemotaxis
47
NBT Assay
48
Phagocytosis
49
Intra cellular killing
Sl. No
TESTS
IHC
50
Tissue processing
51
Microphotography
ICC
52
For any specific marker of interest
53
BANA test
54
BAPNA Test
55
Griess Test
Pharmacy
56
Gel preparation
57
Lyophilization
Extraction of herbal Products
58
Aq. Extract
59
Ethanolic Extract
Preparation of Dosage for dental applications
60
Simple gels
61
In Situ gel
62
Nano particle
63
Analytical method development and validation of API by RP-HPLC
64
Bioanalytical method development and Pharmacokinetic parameter estimation by RP-HPLC
In-vitro diffusion studies
65
Identification test for phytoconstituents in extract
66
Acute and chronic toxicity studies
67
In-vivo animal studies for disease like psorisis, diabetes & cancer
68
Formulation and evaluation of novel drug delivery systems like liposome,
tranformer, ethosome, nanoparticle, metalic nanoparticle &
microsponges
Cell culture
Cell Viability/Cell Cytotoxicity Assay
68
Identification test for phytoconstituents in extract
69
MTT Assay on common cell lines
70
XTT Assay
71
Trypan Blue Assay
72
LDH Assay
73
Alkaline Phosphatase Assay
74
CCK 8 Assay (New)
75
Neutral Red Assay
76
Crystal violet Assay
Apoptosis Studies
77
DAPI
78
Double Staining AO & ETBR
79
Mitochondrial Membrance Potential
80
ROS Generation (New)
81
DNA Fragmentation by Electrophoresis
Fibroblast Culture
82
Periodontal Ligament
83
Gingiva
84
Dental Pulp
85
Deciduous Dental Pulp
86
Buccal Mucosa
87
Granulation Tissue
88
Oral squamous cell carcinoma tissue
Stem Cell Culture
89
Identification (ICC)
90
Characterization
91
Proliferation
92
Lineage differentation: Alizarin Red Assay, Alcian Blue Assay, Oil Red O Assay
93
Epithelial Cell Culture
Sl. No
TESTS
Flow Cytometer Services (New)
94
Apoptosis
95
Cell Cycle Analysis
96
Caspase 3 Expression
97
ROS Generation
98
Mitochondrial Membrance Potential
99
DNA Fragmentation (Tunnel Assay)
100
Cell uptake
101
Cell migration by Scratch Assay
102
Cell migration
103
Stem Cell Identification by RT-PCR
104
3D Culture
Biochemistry
105
Sialic acid
106
Artificial Saliva Preparation
107
DPPH
108
Biochemistry Parameters
109
Protein Estimation
110
Artificial Saliva Preparation
111
SOD
112
Catalase
113
MDA
114
Total Antioxidant activity
115
Reduced glutathione
116
Nitric oxide
117
D-Rom Test
Molecular Biology
118
PCR
119
Conventional PCR
120
Multiplex PCR
121
Real time PCR
122
AP PCR
123
Universal PCR
124
Nested PCR (New)
125
Micro RNA PCR
126
Reverse Transcriptase PCR
127
Genetic Polymorphism, RFLP
128
DNA Cleavage
129
Probe/ Primer design
130
Telomere Study
131
Epigenetics Study
132
LAMP
133
DGGE
134
Checkerboard DNA hybridization
FISH
135
Direct
136
Indirect
137
PNA FISH
| Sl. No | TESTS | Microbiology |
|---|---|
| 1 | Diffusion test for aerobic bacteria/facultative bacteria |
| 2 | Diffusion test for anaerobes |
| 3 | MIC activity for aerobic bacteria/ facultative bacteria |
| 4 | MIC activity for anaerobic bacteria |
| 5 | MBC activity for aerobic bacteria/facultative bacteria |
| 6 | MBC activity for anaerobic bacteria | Time Kill Curve |
| 7 | Aerobic/facultative bacteria for 4 exposure |
| 8 | Anerobic bacteria for 4 exposure | Synergism Studies (New) |
| 9 | Two drug combination |
| 10 | Three drug combination | Antifungal Assay |
| 11 | Diffusion test |
| 12 | MIC for fungi |
| 13 | MBC for fungi |
| 14 | Anti tubercular activity | Culture |
| Total facultative/Aerobic bacteria | |
| 15 | Plate count |
| Culture for individual organisms | |
| 16 | One Bacteria |
| 17 | Two Bacteria |
| 18 | Three Bacteria |
| 19 | More than three bacteria(New) | Anaerobic bacteria |
| 20 | Total Plate count |
| 21 | One Bacteria |
| 22 | Two Bacteria |
| 23 | Three Bacteria |
| 24 | More than three bacteria |
| 25 | Culture for Novel Bacteria not yet cultivable anaerobic bacteria | Biofilm studies |
| 26 | Cyrstal violet assay |
| 27 | Biofilm monospecies |
| 28 | Multi species |
| 29 | Biotic surface |
| 30 | Abiotic surface | Bioflim analysis |
| 31 | Culture |
| 32 | Double staining |
| 33 | Biofilm adhesion |
| 34 | Biofilm penetration | Immunology |
| 35 | ELISA |
| 36 | In-house ELISA |
| 37 | Gelatin Zymography |
| 38 | Western Blotting |
| 39 | Protein Blotting |
| 40 | PAGE |
| 41 | 2-D Gel Electrophoresis |
| 42 | Protein Analysis |
| 43 | Immunofluoresence |
| 44 | Immunohistochemistry |
| 45 | Immunocytochemistry | Neutrophil function tests |
| 46 | Chemotaxis |
| 47 | NBT Assay |
| 48 | Phagocytosis |
| 49 | Intra cellular killing |
| Sl. No | TESTS | IHC |
|---|---|
| 50 | Tissue processing |
| 51 | Microphotography | ICC |
| 52 | For any specific marker of interest |
| 53 | BANA test |
| 54 | BAPNA Test |
| 55 | Griess Test | Pharmacy |
| 56 | Gel preparation |
| 57 | Lyophilization | Extraction of herbal Products |
| 58 | Aq. Extract |
| 59 | Ethanolic Extract | Preparation of Dosage for dental applications |
| 60 | Simple gels |
| 61 | In Situ gel |
| 62 | Nano particle |
| 63 | Analytical method development and validation of API by RP-HPLC |
| 64 | Bioanalytical method development and Pharmacokinetic parameter estimation by RP-HPLC | In-vitro diffusion studies |
| 65 | Identification test for phytoconstituents in extract |
| 66 | Acute and chronic toxicity studies |
| 67 | In-vivo animal studies for disease like psorisis, diabetes & cancer |
| 68 | Formulation and evaluation of novel drug delivery systems like liposome, tranformer, ethosome, nanoparticle, metalic nanoparticle & microsponges | Cell culture | Cell Viability/Cell Cytotoxicity Assay |
| 68 | Identification test for phytoconstituents in extract |
| 69 | MTT Assay on common cell lines |
| 70 | XTT Assay |
| 71 | Trypan Blue Assay |
| 72 | LDH Assay |
| 73 | Alkaline Phosphatase Assay |
| 74 | CCK 8 Assay (New) |
| 75 | Neutral Red Assay |
| 76 | Crystal violet Assay | Apoptosis Studies |
| 77 | DAPI |
| 78 | Double Staining AO & ETBR |
| 79 | Mitochondrial Membrance Potential |
| 80 | ROS Generation (New) |
| 81 | DNA Fragmentation by Electrophoresis | Fibroblast Culture |
| 82 | Periodontal Ligament |
| 83 | Gingiva |
| 84 | Dental Pulp |
| 85 | Deciduous Dental Pulp |
| 86 | Buccal Mucosa |
| 87 | Granulation Tissue |
| 88 | Oral squamous cell carcinoma tissue | Stem Cell Culture |
| 89 | Identification (ICC) |
| 90 | Characterization |
| 91 | Proliferation |
| 92 | Lineage differentation: Alizarin Red Assay, Alcian Blue Assay, Oil Red O Assay |
| 93 | Epithelial Cell Culture |
| Sl. No | TESTS | Flow Cytometer Services (New) |
|---|---|
| 94 | Apoptosis |
| 95 | Cell Cycle Analysis |
| 96 | Caspase 3 Expression |
| 97 | ROS Generation |
| 98 | Mitochondrial Membrance Potential |
| 99 | DNA Fragmentation (Tunnel Assay) |
| 100 | Cell uptake |
| 101 | Cell migration by Scratch Assay |
| 102 | Cell migration |
| 103 | Stem Cell Identification by RT-PCR |
| 104 | 3D Culture | Biochemistry |
| 105 | Sialic acid |
| 106 | Artificial Saliva Preparation |
| 107 | DPPH |
| 108 | Biochemistry Parameters |
| 109 | Protein Estimation |
| 110 | Artificial Saliva Preparation |
| 111 | SOD |
| 112 | Catalase |
| 113 | MDA |
| 114 | Total Antioxidant activity |
| 115 | Reduced glutathione |
| 116 | Nitric oxide |
| 117 | D-Rom Test | Molecular Biology |
| 118 | PCR |
| 119 | Conventional PCR |
| 120 | Multiplex PCR |
| 121 | Real time PCR |
| 122 | AP PCR |
| 123 | Universal PCR |
| 124 | Nested PCR (New) |
| 125 | Micro RNA PCR |
| 126 | Reverse Transcriptase PCR |
| 127 | Genetic Polymorphism, RFLP |
| 128 | DNA Cleavage |
| 129 | Probe/ Primer design |
| 130 | Telomere Study |
| 131 | Epigenetics Study |
| 132 | LAMP |
| 133 | DGGE |
| 134 | Checkerboard DNA hybridization | FISH |
| 135 | Direct | 136 | Indirect | 137 | PNA FISH |